C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01)
Patent
CA 2711289
It is intended to provide a means of highly producing oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains .alpha.-amylase promoter of a microorganism belonging to the strain Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed by this vector to give an oxalate decarboxylase-producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium as described above and then harvesting the oxalate decarboxylase thus produced.
L'invention a pour objet de fournir un moyen de production très élevée d'oxalate décarboxylase qui provient d'un microorganisme. On construit un vecteur plasmidique d'expression recombinante, lequel contient un promoteur d'a-amylase d'un microorganisme qui appartient à la souche Bacillus et un gène d'oxalate décarboxylase qui provient d'un microorganisme qui est obtenu sous le contrôle du promoteur. Ce vecteur transforme une bactérie hôte pour donner une bactérie productrice d'oxalate décarboxylase. Une oxalate décarboxylase recombinante est produite en cultivant la bactérie productrice de la manière décrite ci-dessus et en recueillant ensuite l'oxalate décarboxylase ainsi produite.
Kojima Kenji
Kojima Yuzo
Koyama Takahumi
Minoda Masashi
Amano Enzyme Inc.
Nelligan O'brien Payne Llp
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