C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/26 (2006.01) C12M 1/40 (2006.01) C12Q 1/00 (2006.01) G01N 27/327 (2006.01) G01N 27/416 (2006.01) G01N 27/42 (2006.01)
Patent
CA 2153350
A method and a device for quantifying a substrate in a sample liquid by use of a biosensor are provided. The biosensor can quantify the substrate by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate in the sample liquid and an oxidoreductase. The biosensor includes: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode, and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase. The method includes the steps of: applying a voltage between the counter electrode and the third electrode; supplying the sample liquid to the reaction layer; detecting an electrical change between the counter electrode and the third electrode generated by the supply of the sample liquid to the reaction layer; applying a voltage to the working electrode after the detection using at least the third electrode as a reference; and measuring a current generated between the working electrode and the counter electrode.
Ikeda Shin
Nankai Shiro
Yoshioka Toshihiko
Matsushita Electric Industrial Co. Ltd.
Osler Hoskin & Harcourt Llp
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