Phagemid display system

Details Phagemid display system Phagemid display system

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/73 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2462531

The present invention provides a novel helper phage and phagemid and phagemid display system that comprises an amber mutation in gene 3 of the helper phage so that it is not expressed in the non-permissive bacteria and an in-frame stop codon in the phagemid prior to the gene 3 coding sequence that prevents expression of g3p unless a foreign gene is inserted therein, thus preventing propagation of insert-less phagemids. This results in improved display of foreign gene products on individual virions, avoidance of virions lacking foreign gene inserts and the creation of large phage display libraries.

L'invention porte: sur un nouveau phage assistant; sur un phag~mide; sur un syst­me de pr~sentation du phag~mide qui pr~sente une mutation ambre dans le g­ne 3 du phage assistant qui ainsi n'est pas exprim~ dans la bact~rie non permissive; et sur un codon non-sens encadr~ situ~ dans le phag~mide avant la s~quence codant pour le g­ne 3 qui empÚche l'expression du g3p sauf si y est ins~r~ un g­ne ~tranger qui empÚche la propagation des phag~mides sans insert. Il en r~sulte une pr~sentation am~lior~e des produits g~niques ~trangers sur les virions individuels, l'absence de virions sans inserts de g­nes ~trangers, et la cr~ation de grosses biblioth­ques de pr~sentation de phages.

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