Method, compositions and kit for detection and...

Details Method, compositions and kit for detection and... Method, compositions and kit for detection and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01L 7/00 (2006.01) C12Q 1/70 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2252487

Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenaseTM which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide triphosphate feedstocks, and a chain terminating nucleotide triphosphate. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.

Il est possible de déterminer dans un échantillon la présence et la nature qualitative d'un micro-organisme dans un réceptacle unique en combinant un échantillon à teneur isotopique naturelle contenant de l'ADN avec un mélange de séquençage comprenant une paire d'amorces, une polymérase thermiquement stable telle que la ThermoSéquénase?TM¿ qui incorpore les didésoxynucléotides dans un polymère d'acide nucléique étiré, à une vitesse qui n'est pas inférieure à environ 0,4 fois la vitesse d'incorporation des désoxynucléotides, des charges de nucléotides triphosphates et un nucléotide triphosphate terminant une chaîne. Le traitement de ce mélange s'effectue au cours d'une pluralité de cycles thermiques comprenant les phases de renaturation, d'extension et de dénaturation afin d'obtenir un produit qui est analysé par électrophorèse.

Affiliated with

Also associated with

Rating

Method, compositions and kit for detection and... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method, compositions and kit for detection and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method, compositions and kit for detection and... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1982439