Chimeric promoters capable of mediating gene expression in...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C07K 16/16 (2006.01) C12N 5/10 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2350398

Described are synthetic promoters capable of mediating gene expression in plants upon pathogen infection. Furthermore, recombinant genes and vectors comprising said chimeric promoters as well as host cells transformed with such chimeric promoters, recombinant genes or vectors are provided. Additionally, diagnostic compositions and kits comprising such chimeric promoters, recombinant genes, vectors or cells are described. Provided are further methods for the identification of compounds being capable of activating or inhibiting genes that are specifically expressed in plants upon pathogen infection employing the above described means. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described chimeric promoters, recombinant genes and vectors as well as the use of the aforementioned chimeric promoters, recombinant genes, vectors and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture are described.

L'invention concerne des promoteurs synthétiques capables de servir de médiateurs de l'expression génique dans des végétaux en cas d'infection pathogène. Elle concerne en outre des gènes recombinants et des vecteurs comprenant ces promoteurs chimères ainsi que des cellules hôtes transformées avec ces promoteurs chimères, des gènes recombinants et des vecteurs. L'invention concerne aussi des compositions et kits diagnostiques comprenant ces promoteurs chimères, des gènes recombinants, des vecteurs ou des cellules. Elle concerne en outre des procédés pour identifier les composés capables d'activer ou d'inhiber les gènes qui sont spécifiquement exprimés dans des végétaux lors d'une infection pathogène au moyen des dispositifs décrits plus haut. L'invention concerne enfin des cellules végétales transgéniques, des tissus végétaux et des plantes contenant les promoteurs chimères ci-décrits, les gènes recombinants, les vecteurs et/ou les composés identifiés par le procédé de l'invention dans des cellules végétales et la culture de tissus, en sélection des plantes et/ou en agriculture.

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