Methods for producing transgenic large mammals

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A01K 67/027 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2313702

A transgenic large mammal is produced by a method including the steps of obtaining one ore more early embryos, selectively preparing an embryo having at least three cells, and preferably at a stage in development corresponding in time to the onset of transcription of the embryo's paternal genome, and introducing isolated nucleic acid molecules into a blastomere of the selected embryo. The introduction of isolated nucleic acid molecules into such embryos results in the generation of transgenic large mammals at a significantly increased frequency as compared to introducing isolated nucleic acid molecules into zygotes or into the blastomeres of embryos at the one or two cell stage of development.

On produit un gros mammifère transgénique au moyen d'un procédé consistant à obtenir un ou plusieurs embryons précoces, à préparer de façon sélective un embryon possédant au moins trois cellules et présentant, de préférence, un stade de développement correspondant en temps au début de la transcription du génome paternel de l'embryon, et à introduire des molécules isolées d'acides nucléiques dans une blastomère de l'embryon sélectionné. L'introduction de molécules isolées d'acides nucléiques dans ces embryons permet de générer de gros animaux transgéniques à une fréquence considérablement plus importante si on la compare à l'introduction de molécules isolées d'acides nucléiques dans des zygotes ou dans les blastomères d'embryons au stade de développement d'une ou de deux cellules.

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