Method for producing the mwoi restriction endonuclease and...

Details Method for producing the mwoi restriction endonuclease and... Method for producing the mwoi restriction endonuclease and...

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 15/64 (2006.01) C12N 1/00 (2006.01) C12N 5/16 (2006.01) C12N 9/10 (2006.01) C12N 9/22 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2011975

ABSTRACT The present invention is directed to a method for cloning and producing the MwoI restriction endonuclease by 1) introducing the restriction endonuclease gene from M. wolfel into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the MwoI restriction endonuclease activity, and 3) purifying the MwoI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the MwoI restriction endonuclease activity.

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