Devices and methods to image objects

Details Devices and methods to image objects Devices and methods to image objects

G - Physics

06

K

G06K 9/00 (2006.01) A61B 5/055 (2006.01)

Patent

CA 2431818

Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to automated laser scanning to generate sequential digitized x-y sub-images or partial images of target and non-target objects that are combined to form reconstructed full images, thereby allowing detection, enumeration, differentiation and characterizarion of the imaged objects on the basis of size, morphology and immunophenotype.

L'invention concerne des dispositifs et des procédés destinés à une collecte et à une analyse d'images automatiques, qui permettent l'identification et la classification d'objets microscopiques alignés ou déposés sur des surfaces. Ces objets, p. ex. des cellules cibles rares marquées de façon détectable, sont magnétiquement ou non magnétiquement immobilisés ou soumis à un balayage laser automatisé afin de générer des sous-images ou des images partielles numérisées x-y d'objets cibles ou d'objets non cibles qui sont combinés pour former des images complètes reconstruites, ce qui permet la détection, l'énumération, la différenciation et la caractérisation des objets mis en images sur la base de la taille, de la morphologie et de l'immunophénotype.

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