Preparation of nucleic acid from blood

Details Preparation of nucleic acid from blood Preparation of nucleic acid from blood

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C07H 21/00 (2006.01) C12N 1/06 (2006.01) C12N 5/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C12S 3/22 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2137690

Method and kit for the preparation of nuc- leic acid, for detection and/or for amplification of said nucleic acid. The nucleic acid may be prepared by contacting a sample of cells, particularly whole blood, with an appropriate lysing agent under con- ditions in which red blood cells are lysed but white blood cells are not. The treated sample may then be centrifuged so as to separate desired white blood cells from non-desired red blood cells, and then pu- rifying and lysing said desired white blood cells. The nucleic acid associated with the lysed cells can be detected and/or amplified, and the nucleic acid can also be screened for a certain nucleic acid se- quence, such as a viral nucleic acid sequence. In particular, hemolysis may be performed using eith- er CTAB or saponin, as illustrated in the figure.

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