Methods for defining cell types

Details Methods for defining cell types Methods for defining cell types

C - Chemistry, Metallurgy

12

N

C12N 15/65 (2006.01) C07K 14/435 (2006.01) C12N 5/10 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2311239

The invention provides methods and compositions for defining a cell type, generally involving the steps of: (a) defining a heterogenous subpopulation of cells of an organism; (b) constructing a comprehensive library from the mRNA of the subpopulation of cells; (c) amplifying the mRNA of a single cell of the population; and (d) probing the library with the amplified mRNA to define gene expression of the cell, wherein the gene expression of the cell provides a marker defining the cell type.

L'invention concerne des méthodes et compositions destinés à la définition d'un type cellulaire, les méthodes consistant généralement: (a) à définir une sous-population hétérogène de cellules dans un organisme; (b) à créer une banque complète à partir de l'ARN messager de la sous-population de cellules; (c) à amplifier l'ARN messager d'une cellule unique de la population; et (d) à sonder la banque avec l'ARN messager amplifié pour définir une expression génique de la cellule, l'expression génique de la cellule permettant d'obtenir un marqueur définissant le type cellulaire.

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