C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
47/4, 195/1.2, 1
C12N 15/82 (2006.01) A01H 4/00 (2006.01) C12N 5/14 (2006.01) C12N 15/40 (2006.01) C12N 15/52 (2006.01)
Patent
CA 2037677
A process for production of an exogenous gene or its product in a plant cell which comprises: inserting into a genome of plant, i) cDNA of replicase gene from an RNA plant virus, and ii) eDNA of a recombinant virus genomic RNA in which coat protein gene is wholly or partly replaced with desired exogenous gene, or inoculating said recombinant virus genomic RNA on a plant cell having cDNA of replicase gene inserted in the genome and, iii) allowing the plant to 1) express the replicase genes to produce replicase proteins, 2) replicate the recombinant RNA together with the exogenous gene and 3) express the exogenous gene to produce its expression product. The process of the present invention enables one to efficiently produce the desired exogenous gene product in plant cells in large quantities.
Furusawa Iwao
Mise Kazuyuki
Mori Masashi
Okuno Tetsuro
Fetherstonhaugh & Co.
Furusawa Iwao
Mise Kazuyuki
Mori Masashi
Nihon Nohyaku Co. Ltd.
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