Serum-free media for chondrocytes and methods of use thereof

Details Serum-free media for chondrocytes and methods of use thereof Serum-free media for chondrocytes and methods of use thereof

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12N 5/077 (2010.01) C12N 5/02 (2006.01)

Patent

CA 2473360

The present invention provides defined serum-free cell culture media useful in culturing fibroblasts, especially articular chondrocytes, that avoids problems inherent in the use of serum-containing media. The defined media comprise platelet-derived growth factor (PDGF), and chemically defined lipids, or combinations of these compounds. In another aspect, the present invention also provides tissue culture methods that comprise incubating chondrocytes in the defined serum free media. The methods enhance attachment and proliferative expansion of chondrocytes seeded at low density while maintaining their redifferentiation potential.

L'invention concerne un milieu de culture cellulaire dépourvu de sérum défini, utile dans la culture de fibroblastes, plus spécifiquement de chondrocytes articulaires, lequel milieu permet d'éviter des problèmes inhérents à l'utilisation d'un milieu contenant du sérum. Ce milieu défini comprend un facteur de croissance dérivé des plaquettes (PDGF) et des lipides définis chimiquement, ou des combinaisons de ces composés. Dans un autre mode de réalisation, l'invention concerne également des procédés de culture tissulaire qui comprennent l'incubation de chondrocytes dans le milieu dépourvu de sérum défini. Ces procédés permettent de renforcer la fixation et l'expansion proliférante de chondrocytes ensemencés à une faible densité, tout en conservant le potentiel de redifférentiation de ces derniers.

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