Use of gene ncsag4 for the diagnosis and prevention of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/30 (2006.01) C07K 14/44 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2548582

The invention relates to gene NcSAG4, the RNA messenger of said gene, the oligonucleotides designed as a function of the nucleotide sequence thereof or any of the fragments of same, and to protein NcSAG4 coded thereby, or any of the recombinant forms of same, expression vectors, and host cells containing same, for the purpose of diagnosis and vaccination for the prevention of neosporosis. The invention also relates to the use thereof as a specific marker of the N. caninum bradyzoite stage, for the analysis of the pathogenesis or the effectiveness of vaccines against the establishment of a chronic infection in the intermediate host.

L'invention concerne le gène Nc<I>SAG</I>4, son ARN messager, les oligonucléotides conçus en fonction de sa séquence nucléotidique, ou un quelconque de ses fragments, la protéine Nc<I>SAG</I>4 codée par ledit gène, ou une quelconque de ses formes recombinées, les vecteurs d'expression, les cellules hôtes qui les contiennent, à des fins diagnostiques et de vaccination pour la prévention contre une infection par Neospora, et son utilisation en tant que marqueur spécifique de la phase de bradyzoïte de N. caninum, pour l'analyse de la pathogénie ou de l'efficacité des vaccins lorsqu'une infection chronique est observée chez l'hôte intermédiaire.

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