Method for proliferating cardiomyocytes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) A61K 31/7088 (2006.01) A61K 35/34 (2006.01) A61K 35/76 (2006.01) A61K 48/00 (2006.01) A61P 9/00 (2006.01) A61P 9/04 (2006.01) A61P 9/10 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2545049

The growth of myocardial cells is induced by expressing cycline and CDK in the myocardial cells and regulating the function or action of a Cip/Kip protein, or by inhibiting the production of a Cip/Kip protein. Among the Cip/Kip family proteins, it is preferable to regulate the function of p27Kip1 or inhibiting the production thereof. As a recombinant vector to be used therefor, it is intended to provide a vector having: (1) a cyclin gene; (2) a cyclin-dependent kinase gene and (3) one or more members selected from the group consisting of a gene encoding a factor inhibiting the function or action of a Cip/Kip protein and nucleic acid sequences inhibiting the production of Cip/Kip family proteins.

La culture de cellules myocardiques est induite par l'expression de la cycline et de CDK dans les cellules myocardiques et par la régulation de la fonction ou de l'action d'une protéine Cip/Kip, ou par l'inhibition de la production d'une protéine Cip/Kip. Parmi les protéines de la famille Cip/Kip, il est préférable de réguler les fonctions de p27?kip1¿ ou d'inhiber sa production. Le vecteur de recombinaison à utiliser à cet effet, est un vecteur possédant: (1) un gène de cycline; (2) un gène de kinase dépendant de la cycline et(3) un ou plusieurs éléments choisis dans le groupe formé d'un gène codant un facteur inhibant la fonction ou l'action d'une protéine Cip/Kip et des séquences nucléotidiques inhibant la production de protéines de la famille Cip/Kip.

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