Process for the production of unfused protein in e. coli

Details Process for the production of unfused protein in e. coli Process for the production of unfused protein in e. coli

C - Chemistry, Metallurgy

12

N

C12N 15/18 (2006.01) A61K 38/30 (2006.01) C07K 14/65 (2006.01) C12N 1/21 (2006.01) C12N 15/17 (2006.01) C12N 15/73 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2030947

4-17838/= Novel process for the production of unfused protein in E. coli Abstract of the Disclosure A process for the preparation of a recombinant IGF-II (rIGF-II) without a covalently attached foreign protein moiety and without N-terminal attached methionine or a deriva- tive of methionine or of a salt of said IGF-II, rIGF-II produced by said method, hybrid vectors comprising DNA encoding said rIGF-II, hosts transformed with said vectors, and a process for the isolation of said rIGF-II from the host cell and refolding it into a biologically active form.

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