Methods and compositions for accelerating the generation of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0783 (2010.01) A61K 35/14 (2006.01) A61P 37/06 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2721088

The present invention is directed to generating regulatory T cells by treating a cell culture that includes non-regulatory T cells with a regulatory composition. The invention encompasses methods utilizing a regulatory composition that includes agents that prevent methylation of the locus for the FOXP3 transcription factor, agents that accelerate differentiation of T cells into suppressor cells, and agents that are histone deacetylase inhibitors. The invention also encompasses compositions of regulatory T cells generated by cultuhng non-regulatory T cells with a regulatory composition as well as the use of such regulatory T cells in the treatment of autoimmune diseases and aberrant immune responses.

La présente invention concerne la génération de cellules T régulatrices par traitement d'une culture cellulaire qui comprend des cellules T non-régulatrices avec une composition régulatrice. L'invention concerne des procédés d'utilisation d'une composition régulatrice qui comprend des agents qui empêchent une méthylation du lieu pour le facteur de transcription FOXP3, des agents qui accélèrent une différenciation des cellules T dans des cellules suppresseurs, et des agents qui sont des inhibiteurs dhistone déacétylase. L'invention concerne également des compositions de cellules T régulatrices générées par culture de cellules T non-régulatrices avec une composition régulatrice, de même que l'utilisation de telles cellules T régulatrices dans le traitement de maladies auto-immunes et de réponses immunitaires aberrantes.

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