C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) C07H 21/00 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 15/64 (2006.01) C12N 15/72 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2138399
A method of cloning of xylanase clones from an anaerobic rumen fungus including the steps of: (i) cultivation of an anaer- obic rumen fungus; (ii) isolating total RNA from the culture in step (i); (iii) isolating poly A + mRNA from the total RNA re- ferred to in step (ii); (iv) constructing a cDNA expression library; (v) ligating cDNA to a bacteriophage expression vector selected from .lambda.ZAP, .lambda.ZAPII or vectors of similar properties; (vi) screening of xylanase positive recombinant clones in a culture medium incorporating xylan by detection of xylan hydrolysis; and (vii) purifying xylanase positive recombinant clones. There is also provided xylanase positive recombinant clones produced by the above-mentioned method as well as xylanase positive recom- binant clones having the following properties: (i) production of xylan clearing zones in a culture containing xylanase cDNA de- rived from N. patriciarum; (ii) having activity in hydrolysis of xylan but having no activity in relation to hydrolysis of CMC or crystalline cellulose. There is also provided various cDNA molecules which may be utilised in the above-mentioned method.
Commonwealth Scientific And Industrial Research Organisation
Ridout & Maybee Llp
LandOfFree
Recombinant xylanase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant xylanase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant xylanase will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1995998