Novel anti-vh3-15 reagents, vh3-15 polypeptides, cell...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/13 (2006.01) C07H 21/00 (2006.01) C07K 14/705 (2006.01) C07K 16/12 (2006.01) C07K 16/28 (2006.01) C07K 16/42 (2006.01) C12N 5/18 (2006.01) C12N 15/12 (2006.01) C12Q 1/68 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2200329

The present invention provides novel anti-VH3-15 idiotypic antibody materials and hybridomas producing this antibody material. Also provided in the present invention are VH3-15 autoantibodies recognized by anti-VH3-15 idiotypic antibody material and the cell surface antigens which bind VH3-15 autoantibody. The anti-VH3-15 idiotypic antibody material, VH3-15 autoantibodies and cell surface antigens of the present invention can be used in methods for detecting, isolating and purifying VH3-15 polypeptides and in diagnostic assays for IBD and infection by Campylobacter jejuni. Nucleic acid molecules encoding anti-VH3-15 antibody material, VH3-15 autoantibody and cell surface antigens, as well as methods for their use are also provided.

L'invention concerne de nouveaux matériaux d'anticorps idiotypiques anti-VH3-15, ainsi que des hybridomes produisant ces matériaux. Elle concerne également des auto-anticorps de VH3-15 reconnus par le matériau d'anticorps idiotypique anti-VH3-15, ainsi que les antigènes de surface de cellule se fixant à l'auto-anticorps de VH3-15. Ce matériau d'anticorps idiotypique anti-VH3-15, les auto-anticorps de VH3-15 et les antigènes de surface de cellule peuvent s'utiliser dans des procédés de détection, d'isolation et de purification de polypeptides de VH3-15, ainsi que dans des procédés servant à effectuer le diagnostic de colites inflammatoires et d'infection par Campylobacter jejuni. L'invention concerne également des molécules d'acide nucléique codant le matériau d'anticorps anti-VH3-15, l'auto-anticorps de VH3-15 et les antigènes de surface de cellule, ainsi que des procédés servant à les utiliser.

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