Assembly of wild-type and chimeric influenza virus-like...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/44 (2006.01) A61K 39/145 (2006.01) C07K 14/11 (2006.01) C12N 7/04 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2410297

Influenza virus-like particles (VLPs) comprising the structural proteins HA, NA, M1 and M2 are described. VLPs are also generated containing M1 alone, as are VLPs with M1 and any one or two of HA, Na and M2. VLPs with HA from one influenza subtype and NA from a different influenza subtype are also described, as are VLPs in which a portion or all of HA or NA is replaced by a heterologous moiety not produced by influenza virus, so as to comprise chimeric VLPs.

La présente invention concerne des particules viraloïdes (VLP) de la grippe comprenant les protéines structurelles HA, NA, M1 et M2. On a également généré des VLP contenant la MA seule, ainsi que des VLP avec la M1 et une ou deux des protéines HA, NA, et M2. L'invention concerne également des VLP avec une HA d'un sous-type de la grippe, et une NA d'un sous-type différent de la grippe, mais aussi des VLP dans lesquelles tout ou partie des HA et NA est remplacé par un fragment hétérologue non produit par le virus de la grippe, de façon à englober les VLP chimériques.

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