In vitro maturation and fertilization of mammalian oocytes

Details In vitro maturation and fertilization of mammalian oocytes In vitro maturation and fertilization of mammalian oocytes

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2199663

An in vitro fertilization method comprising culturing mammalian immature oocytes obtained from a mammalian ovary very early in the mammal's menstrual cycle in an oocyte maturation inhibitor-containing culture, removing the inhibitor and maturing and fertilizing the oocytes to produce embryos for subsequent uterine- implantation. The method allows for more convenient, efficacious, safer, less expensive and timely implantation of a plurality of embryos in the uterus to enhance pregnancy success rate. Cryopreservation of the immature oocytes offers enhanced benefits Preferably, the mammal is human and the inhibitor is dbcAMP.

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