Detection of nucleic acids in cells by thermophilic strand...

Details Detection of nucleic acids in cells by thermophilic strand... Detection of nucleic acids in cells by thermophilic strand...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2305858

Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.

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