C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/02 (2006.01) C12Q 1/26 (2006.01) C12Q 1/44 (2006.01) C12Q 1/527 (2006.01) G01N 33/52 (2006.01) G01N 33/567 (2006.01) G01N 33/573 (2006.01)
Patent
CA 2519259
The invention relates to methods for the quantitative optical analysis of the intracellular concentration of the cyclic nucleotides cGMP and cAMP, said methods using cell lines which express a combination of certain CNG channels, a calcium-sensitive photoprotein, and different target proteins for which modulators are to be found, in a recombinant manner. The cell lines modified in this way are suitable for high-throughput screening (HTS and uHTS) and can be used to identify medicaments which influence the activity of receptors or enzymes participating in the composition or decomposition of the cyclic nucleotides cGMP and cAMP.
L'invention concerne un procédé destiné à l'analyse optique quantitative de la concentration intracellulaire des nucléotides cycliques cGMP cAMP. S'avèrent à cet effet utiles, les lignées cellulaires qui expriment de manière recombinante une combinaison de certains canaux CNG, une photoprotéine sensible au calcium et différentes protéines cibles pour lesquelles des modulateurs doivent être cherchés. Les lignées cellulaires ainsi modifiées sont adaptées au dépistage haute capacité (HTS et uHTS) et peuvent servir à l'identification de médicaments qui agissent sur l'activité de récepteurs ou enzymes participant à l'élaboration ou à la décomposition des nucléotides cycliques.
Axxam S.p.a.
Bayer Healthcare Ag
Bayer Schering Pharma Aktiengesellschaft
Fetherstonhaugh & Co.
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