Optical quantification of analytes in membranes

Details Optical quantification of analytes in membranes Optical quantification of analytes in membranes

G - Physics

01

N

G01N 33/543 (2006.01) G01N 21/75 (2006.01) G01N 33/558 (2006.01)

Patent

CA 2267126

Methods for measuring the amount of an analyte in a membrane, comprising applying to the membrane a clearing agent, are disclosed. The clearing agent can be an agent that has approximately the same refractive index as the membrane; alternatively, the clearing agent can be a dissolving agent, that dissolves the membrane. The analyte can be labelled to facilitate detection. Representative labels include fluorescent labels and detectable particles.

L'invention concerne des procédés de mesure de la quantité d'une substance à analyser présente dans une membrane, qui consistent à appliquer sur la membrane un agent éclaircissant. L'agent éclaircissant peut être un agent qui a approximativement le même indice de réfraction que la membrane. Dans un autre mode de réalisation, l'agent de clarification peut être un agent dissolvant, qui dissout la membrane. La substance à analyser peut être marquée pour faciliter sa détection, notamment par des marqueurs fluorescents ou des particules détectables.

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