Method for producing polyunsaturated fatty acids, novel...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01K 67/00 (2006.01) A61K 31/20 (2006.01) C07K 16/40 (2006.01) C11B 1/00 (2006.01) C12N 9/02 (2006.01) C12N 15/11 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01) C12N 15/85 (2006.01) C12P 7/62 (2006.01) C12P 7/64 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2435081

The invention relates to a method for producing unsaturated fatty acids comprising at least two double bonds, and a method for producing triglycerides having an increased content of polyunsaturated fatty acids comprising at least two double bonds. The invention also relates to the advantageous use of the nucleic acid sequences of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11 or SEQ ID NO: 18 in the inventive methods, and for producing a transgenic organism, preferably a transgenic plant or a transgenic micro- organism having an increased content of fatty acids, oils or lipids containing unsaturated C18, C20, or C22 fatty acids. The invention further relates to novel desaturases comprising the sequences of SEQ ID NO. 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11, or the homologues, derivatives or analogues thereof, and gene constructs comprising said genes or the homologues, derivatives or analogues thereof, in addition to the use of the same, either alone or combined with biosynthesis genes of polyunsaturated fatty acids, as advantageously represented in SEQ ID NO: 7 and SEQ ID NO: 9. Furthermore, the invention relates to isolated nucleic acid sequences, expression cassettes containing said nucleic acid sequences, vectors, and transgenic organisms containing at least one nucleic acid sequence or an expression cassette. The invention also relates to unsaturated fatty acids comprising at least two double bonds and triglycerides having an increased content of unsaturated fatty acids comprising at least two double bonds, and the use of the same. Finally, the invention relates to multi-expression cassettes for seed-specific expression, in addition to vectors or organisms comprising a desaturase gene, either alone or combined with other desaturases having the sequence of SEQ ID NO: 7 and/or elongase genes having the sequence of ID NO: 9 or the homologues, derivatives or analogues of the same, expressed by means of the above- mentioned expression cassettes.

La présente invention concerne un procédé de production d'acides gras comprenant au moins deux doubles liaisons et/ou un procédé de production de triglycérides présentant une teneur accrue en acides gras polyinsaturés comprenant au moins deux doubles liaisons. L'invention concerne également l'utilisation avantageuse des séquences d'acides nucléiques SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11 ou SEQ ID NO: 18 dans ces procédés et pour produire un organisme transgénique, de préférence une plante transgénique ou un microorganisme transgénique présentant une teneur accrue en acides gras, huiles ou lipides comprenant des acides gras C¿18?, C¿20? ou C¿22? insaturés. L'invention concerne en outre de nouvelles désaturases comprenant les séquences SEQ ID NO. 1, SEQ ID NO: 3, SEQ ID NO: 5 et SEQ ID NO: 11 ou des homologues, dérivés ou analogues, ainsi que des constructions géniques contenant ces gènes ou leurs homologues, dérivés ou analogues, de même que leur utilisation seules ou en combinaison avec des gènes biosynthétiques d'acides gras polyinsaturés, représentés avantageusement dans les séquences SEQ ID NO: 7 et SEQ ID NO: 9. L'invention concerne par ailleurs des séquences isolées d'acides nucléiques, des cassettes d'expression contenant ces séquences d'acides nucléiques, des vecteurs et des organismes transgéniques contenant au moins une séquence d'acides nucléiques ou une cassette d'expression. L'invention concerne également des acides gras insaturés comprenant au moins deux doubles liaisons et des triglycérides présentant une teneur accrue en acides gras insaturés comprenant au moins deux doubles liaisons, ainsi que leur utilisation. L'invention concerne enfin des cassettes de multi-expression permettant l'expression spécifique à des semences, ainsi que des vecteurs ou des organismes qui comprennent un gène de désaturase seul ou en combinaison avec d'autres désaturases comprenant la séquence SEQ ID NO: 7 et/ou des gènes d'élongase comprenant la séquence ID NO: 9 ou des homologues, dérivés ou analogues, exprimés au moyen des cassettes d'expression mentionnées.

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