Deformylation of f-met peptides in bacterial expression systems

Details Deformylation of f-met peptides in bacterial expression systems Deformylation of f-met peptides in bacterial expression systems

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C07K 14/61 (2006.01) C12N 1/21 (2006.01) C12N 9/80 (2006.01) C12N 15/55 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2261016

A method for producing recombinant peptides and proteins having a diminished retention of N-formyl-methionine by coexpressing a peptide deformylase enzyme is disclosed. Also disclosed are substantially deformylated protein compositions and transformed bacterial cells and DNA vectors useful for acheiving such deformylation.

La présente invention concerne un procédé de production de peptides et protéines de recombinaison caractérisés par une moindre rétention de la N-formyl-méthionine grâce à une coexpression d'une enzyme déformylase de peptide. L'invention concerne également des compositions de protéines sensiblement déformylées et des cellules bactériennes transformées ainsi que des vecteurs ADN convenant à la réalisation d'une telle déformylation.

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