Methods and compositions for modulating lipid content of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/245 (2006.01) C11B 1/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2166625

Novel compositions and methods are provided for modifying the lipid content of plant tissues of interest, including leaf, root, fruit and seed. The methods involve transforming a plant cell of interest with an expression cassette functional in a plant cell comprising a transcriptional and translational initiation regulatory region, joined in reading frame 5' to a DNA sequence encoding an enzyme capable of modulating the production of fatty acids, and translational and transcriptional termination regions. Expression of the enzyme provides for an increase in fatty acid biosynthesis as a result of altered concentrations of substrate for enzymes involved in fatty acid biosynthesis. Of particular interest is the selective control of lipid production in plant tissues such as leaves, root, fruit and seed.

L'invention concerne des nouvelles compositions et des procédés de modification de la teneur en lipides des tissus d'intérêt de végétaux, y compris les feuilles, les racines, les fruits et les graines. Le procédé consiste à transformer une cellule d'intérêt de plante avec une cassette d'expression fonctionnelle dans une cellule de plante comprenant une région régulatrice d'initiation transcriptionnelle et translationnelle, réunie dans la phase de lecture 5' à une séquence d'ADN codant une enzyme capable de moduler la production d'acides gras, et les régions de terminaison translationnelles et transcriptionnelles. L'expression de l'enzyme génère un accroissement dans la biosynthèse d'acides gras du fait des concentrations modifiées du substrat pour les enzymes impliquées dans la biosynthèse d'acides gras. La régulation sélective de la production lipidique des tissus de végétaux, tels que les feuilles, les racines et les fruits et les graines présente un intérêt particulier.

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