Method for identifying nucleic acids by electro-spray mass...

Details Method for identifying nucleic acids by electro-spray mass... Method for identifying nucleic acids by electro-spray mass...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2312945

The invention relates to a method for determining a nucleotide sequence in a nucleic acid molecule by means of predetermined probes of different masses, using electro-spray mass spectrometry. The inventive method enables simultaneous characterisation of a plurality of unknown nucleic acid molecules by means of a set of various probes. The invention also relates to a kit containing the probes and/or a probe carrier, optionally with nucleic acid molecules bonded thereto.

La présente invention concerne un procédé de détermination d'une séquence nucléotidique dans une molécule d'acide nucléique, au moyen de sondes prédéterminées de masses différentes, par spectrométrie de masse par électropulvérisation. De façon avantageuse, le procédé selon l'invention permet la caractérisation simultanée d'une pluralité de molécules d'acide nucléique inconnues au moyen d'un jeu de sondes différentes. L'invention concerne en outre un nécessaire contenant les sondes et/ou un porte-échantillons, auxquels sont éventuellement liées des molécules d'acide nucléique.

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