Recombinant virus production for the manufacturing of vaccines

Details Recombinant virus production for the manufacturing of vaccines Recombinant virus production for the manufacturing of vaccines

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C07K 14/075 (2006.01) C12N 7/04 (2006.01) C12N 15/86 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2501975

The present invention relates to the production of recombinant viruses and/or recombinant viral proteins using cells that can grow in suspension and in serum-free conditions without the requirement of any animal- or human derived components. In particular, the invention relates to the production of recombinant alphaviruses that are suitable for use in vaccines and in gene therapy applications. More in particular, Semliki Forest Virus (SFV) particles carrying a heterologous gene of interest (e.g., an antigen) are produced on E1- transformed non tumorous human cells, preferably derived from primary retinoblasts, such as PER.C6TM cells.

La présente invention concerne la production de virus recombinés et/ou de protéines virales recombinées à partir de cellules pouvant se développer dans une suspension et dans un milieu de culture exempt de sérum sans qu'il faille utiliser des composantes dérivées d'animaux ou d'humains. L'invention concerne plus particulièrement la production d'alphavirus recombinés pouvant être utilisés dans des vaccins et en thérapie génique. L'invention concerne plus précisément la production de particules d'un virus de la Forêt de Semliki (SFV) transportant un gène hétérologue étudié (un antigène, par exemple) sur des cellules humaines non tumorales transformées par E1, de préférence dérivées de rétinoblastes primaires, telles que des cellules PER.C6.TM..

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