Kit and method for determining hla types

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12M 1/00 (2006.01) C12N 15/09 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2413931

The genotype of a test sample HLA is determined by using a substrate, on which oligonucleotides of 10 to 24 bases in length originating in the sequences of genes belonging to the HLA class I antigens or class II antigens on human genome and containing gene polymorphisms as alloantigens in these sequences have been immobilized, and hybridizing with a nucleic acid sequence originating in the test sample.

L'invention concerne un procédé de détermination du génotype d'un échantillon d'antigène HLA, consistant à utiliser un substrat, sur lequel on immobilise des oligonucléotides de 10 à 24 bases provenant des séquences de gènes qui appartiennent aux antigènes HLA de classe I ou II du génome humain et présentant des polymorphismes génétiques en tant qu'alloantigènes dans lesdites séquences, ainsi qu'à produire un hybride présentant une séquence d'acide nucléique provenant du même échantillon.

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