Mutant proteinase with reduced self-cleavage activity and...

Details Mutant proteinase with reduced self-cleavage activity and... Mutant proteinase with reduced self-cleavage activity and...

C - Chemistry, Metallurgy

12

N

C12N 9/50 (2006.01) C07K 1/36 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2435381

The present invention provides a mutant 27 kDa NIa proteinase having reduced self-cleavage activity relative to the self-cleavage activity of its wild-type proteinase. The mutant has the same substrate cleavage activity as the wild- type proteinase but is more stable than the wild-type proteinase. The present invention also provides a method of obtaining large quantities of active 27 kDa NIa proteinase for use as a tool for purification of other proteins.

La présente invention concerne une protéinase mutante 27 kDa NIa présentant une activité auto-clivante réduite par rapport à l'activité auto-clivante de sa protéinase de type sauvage. La protéinase mutante présente la même activité de clivage du substrat que la protéinase de type sauvage, mais elle est plus stable que la protéinase de type sauvage. L'invention concerne également un procédé permettant d'obtenir de grandes quantités de protéinase active 27 kDa NIa pour une utilisation comme outil de purification d'autres protéines.

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