Ehrlichia canis 120-kda immunodominant antigenic protein and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 39/02 (2006.01) A61P 31/04 (2006.01) C07K 14/29 (2006.01) C07K 16/12 (2006.01) C12N 15/63 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2340462

The present invention provides a 120-kDa protein gene of Ehrlichia canis, amplified by PCR using primers derived from the DNA sequences flanking the Ehrlichia chaffeensis 120-kDa protein gene. The recombinant E. canis 120-kDa protein contains 14 tandem repeat units with 36 amino acids each. The repeat units are hydrophilic and predicted to be surface-exposed. Also disclosed is that the recombinant E. canis 120-kDa protein is antigenic and reacts with sera from dogs convalescent from canine ehrlichiosis.

La présente invention concerne un gène de la protéine de 120 kDa d'Ehrlichia canis, amplifié par amplification en chaîne par polymérase au moyen d'amorces dérivées des séquences d'ADN flanquant le gène de la protéine de 120 kDa d'Ehrlichia chaffeensis. La protéine recombinée de 120 kDa d'E. canis contient 14 séquences répétées en tandem comprenant chacune 36 acides aminés. Les séquences répétées sont hydrophiles et prévues pour être exposées à la surface. L'invention concerne également la découverte que la protéine recombinée de 120 kDa d'E. canis est antigénique et entre en réaction avec du sérum provenant de chiens convalescents ayant souffert d'ehrlichiose canine.

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