Secreted renilla luciferase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12Q 1/48 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2341685

A polynucleotide encoding a secreted form of wild type Renilla Iuciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.

On décrit un polynucléotide codant une forme sécrétée de luciférase Renilla du type sauvage. On décrit également un polynucléotide codant une forme modifiée sécrétée de luciférase Renilla du type sauvage; les polypeptides codés par les polynucléotides de la présente invention et les utilisations de ces mêmes polynucléotides et polypeptides dans des dosages biologiques; ainsi qu'une lignée stable de cellules d'enrobage de mammifère qui produit des rétrovirus portant un polynucléotide codant une luciférase Renilla sécrétée.

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