Novel glucose dehydrogenase and method for producing the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12Q 1/32 (2006.01)

Patent

CA 2427031

A novel glucose dehydrogenase, which is an enzyme having a high substrate specificity, being economically produced, being free from any effect of dissolved oxygen in a sample and having a particularly high heat stability, can be obtained by culturing a microorganism belonging to the genus Burkholderia and being capable of producing glucose dehydrogenase and then collecting the glucose dehydrogenase from the culture medium and/or the microbial cells.

L'invention porte sur une nouvelle glucose déshydrogénase qui est une enzyme présentant un substrat hautement spécifique, produite à faibles coûts et exempte de tout effet d'oxygène dissous dans un échantillon, et ayant notamment une haute stabilité à la chaleur. Le procédé de production de cette enzyme consiste à mettre en culture un micro-organisme appartenant au genre Burkholderia et capable de produire une glucose déshydrogénase, et récupérer ensuite la glucose déshydrogénase dans le milieu de culture et/ou dans les cellules microbiennes.

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