C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/61 (2006.01) C07K 1/30 (2006.01) C07K 1/34 (2006.01)
Patent
CA 2338319
Protein suspensions comprising soluble and insoluble components are purified via flocculation with anionic polymers, such as polyacrylamides, potato starch, or modified cellulose. The procedure improves the efficiency of solid/liquid separations and can minimize or eliminate the requirement for centrifugation and/or filtration in large scale biotechnological processes. The method is particularly well suited for the purification and clarification of protein suspensions containing soluble somatotropin monomer using polyacrylamide and polysaccharide flocculants.
Des suspensions de protéines comprenant des composants solubles et insolubles sont purifiées par floculation avec des polymères anioniques, tels que des polyacrylamides, de l'amidon de pomme de terre ou de la cellulose modifiée. Le procédé améliore le rendement des séparations solides/liquides, et peut minimiser, voire, éliminer complètement les opérations de centrifugation et/ou de filtration habituellement requises dans les procédés biotechnologiques à échelle industrielle. Le procédé est particulièrement bien approprié pour la purification et la clarification de suspensions de protéines à teneur en monomères de somatotropine soluble au moyen de floculants polyacrylamides et polysaccharides.
Mcfadden Fincham
Monsanto Company
Monsanto Technology Llc
LandOfFree
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