Expression system for membrane proteins

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/70 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2387180

The invention relates to a method for preparing a polypeptide comprising: expressing said polypeptide in a host cell for use in protein expression, wherein said host cell has an altered membrane composition, isolating the membrane fraction from said host cell, and isolating said polypeptide from said membrane fraction.

L'invention concerne un procédé de préparation d'un polypeptide, qui comporte les étapes consistant à : exprimer le polypeptide dans une cellule hôte s'utilisant dans l'expression de protéines, cette cellule hôte présentant une composition de membrane modifiée ; isoler la fraction de membrane de ladite cellule hôte ; et isoler ledit polypeptide de cette fraction de membrane.

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