Miniaturized cell array methods and apparatus for cell-based...

G - Physics – 01 – N

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G01N 15/14 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) C12M 1/34 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2345151

The present invention devices and methods for maximizing the number of wells that can be imaged at one time while still obtaining adequate pixel resolution in the image. This result has been achieved through the use of fluidic architectures that maximizes well density. The present invention thus provides a miniaturized microplate system with closed fluidic volumes that are internally supplied with fluid exchange, and with wells that are closely spaced to more rapidly detect spatially-resolved features of individual cells.

La présente invention concerne des dispositifs et méthodes permettant d'accroître le nombre de cupules pouvant être représentées en une seule fois tout en réalisant une définition de pixels appropriée dans l'image. On a obtenu ce résultat grâce à l'utilisation d'architectures fluidiques qui accroissent la densité des cupules. La présente invention concerne donc un système de microplaques miniaturisées comportant des volumes fluidiques fermés alimentés de façon interne par échange de fluides, et des cupules rapprochées pour une détection rapide de caractéristiques à définition spatiale de cellules individuelles.

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