Method for measurement of the activity of nitric-oxide synthase

Details Method for measurement of the activity of nitric-oxide synthase Method for measurement of the activity of nitric-oxide synthase

C - Chemistry, Metallurgy

12

Q

C12Q 1/527 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2417076

The invention relates to a method for measuring the activity of nitric oxide synthase and a corresponding method for identifying nitric oxide synthase modulators wherein the method is a method of measuring a nitric oxide (NO) synthase activity, comprising the steps of: (a) incubating NO synthase in incubation medium with labeled arginine as substrate in a reaction mixture contained in a reaction vessel, whereby arginine is converted into citrulline by the NO synthase, (b) separating nonconverted arginine from the reaction mixture and capturing non-converted arginine by means of a filter plate membrane; and (c) measuring the amount of captured arginine on the filter plate membrane as an indication of the NO synthase activity.

L'invention concerne un procédé pour mesurer l'activité de la NO synthase, un procédé correspondant pour l'identification de modulateurs de NO synthase, en particulier pour application dans une sélection à haut rendement (HTS), et l'utilisation des membranes à plaque filtrante échangeuses de cations correspondantes.

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