High resolution typing system for pathogenic e. coli

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2468842

Variable number tandem repeat (VNTR) sequences have been identified in the genome of certain E. coli O157:H7 strains. It has been discovered that the VNTR sequences exhibit length polymorphism at different loci. A sub-typing system based on multiloci size analysis of VNTR is the basis of the novel molecular sub-typing system of the present invention.

L'invention porte sur des séquences de répétition en tandem, en nombre variable (VNTR), qui ont été identifiées dans le génome de certaines souches O157:H7 de E. coli. On a découvert que ces séquences présentent un polymorphisme de longueur à différents loci. Un sous-système de typage basé sur une analyse de la taille de plusieurs loci des séquences VNTR est la base du nouveau sous-système de typage moléculaire de cette invention.

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