C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12M 3/00 (2006.01)
Patent
CA 2460206
The invention provides a method for production of polypeptides in eukaryote microcarrier cell culture, the method comprising the steps of (i) Culturing cells expressing said polypeptide on microcarriers under conditions and at a setpoint temperature appropriate for expression of said polypeptide; (ii) Cooling the culture to a predetermined temperature below said setpoint; (iii) Sedimenting the microcarriers; and (iv) Harvesting all or part of the culture medium.
La présente invention concerne une technique de production de polypeptides dans des cultures de cellules à billes microporteuses eucaryotes. Cette technique consiste (i) à cultiver des cellules exprimant ce polypeptide sur des billes microporteuses dans des conditions et à un point fixé de température approprié pour l'expression de ce polypeptide, (ii) à refroidir cette culture à une température prédéterminée en dessous du point fixé, (iii) à déposer ces billes microporteuses en couche sédimentaire et (iv) à recueillir tout ce milieu de culture ou une partie de celui-ci.
Dimock Stratton Llp
Novo Nordisk Health Care Ag
LandOfFree
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