System and process for carrying out and monitoring...

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 19/34 (2006.01) B01L 3/14 (2006.01) B01L 7/00 (2006.01) C12M 1/34 (2006.01) C12M 1/38 (2006.01) C12M 1/40 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 21/03 (2006.01) G01N 21/25 (2006.01) G01N 21/64 (2006.01) G01N 21/07 (2006.01) G01N 35/00 (2006.01) G01N 35/02 (2006.01)

Patent

CA 2256773

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Monitoring of the DNA amplification is monitored by fluorescence once per cycle or many times per cycle. The present invention provides that fluorescence monitoring of PCR is a powerful tool for DNA quantification.

L'invention concerne un procédé et un dispositif pour cycle thermique. Le dispositif comprend une chambre à échantillons, dont la température est modulable de manière rapide et précise selon la gamme des températures nécessaires à l'exécution de différentes opérations biologiques (par exemple, amplification en chaîne par ADN polymérase). Placés dans des tubes microcapillaires en verre, les échantillons biologiques sont placés dans la chambre. La régulation de la température de l'échantillon dans cette chambre est assurée par un dispositif programmable. Le contrôle de l'amplification d'ADN est assuré par fluorescence, une ou plusieurs fois par cycle. Au fin de l'invention, il est établi que le contrôle par fluorescence de l'ACP est un outil puissant pour la quantification de l'ADN.

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