Microbially derived enzymes having enhanced milk clotting...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/58 (2006.01) A23C 19/032 (2006.01)

Patent

CA 2206864

Rhizomucor miehei derived aspartic proteases having enhanced milk clotting activity are obtained by deglycosylating the proteases or by producing them in microbial host species which do not glycosylate the proteases or which glycosylate them to lesser extend than Rhizomucor miehei strains naturally producing aspartic proteases in a glycosylated form. Such improved proteases are useful in cheese manufacturing.

On obtient des protéases aspartiques dérivées de rhizomucor miehei et présentant une activité de coagulation du lait accrue, en déglycosylant les protéases ou en les produisant dans des espèces-hôtes microbiennes qui ne glycosylent pas les protéases ou qui les glycosylent dans des proportions moindres que les souches de rhizomucor miehei produisant naturellement des protéases aspartiques sous forme glycosylée. Ces protéases améliorées sont utiles dans la fabrication du fromage.

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