Process for preparing purified nucleic acid and the use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C07H 1/08 (2006.01)

Patent

CA 2245576

The invention relates to a nucleic acid preparation with a content of below 1 % protein, preferably below 0.1 % protein, free of ethidium bromide, phenol, caesium chloride and detergents based on octyl phenol poly(ethylene glycol ether)n and with a content of below 1 EU/mg DNA of endotoxins. Said preparation is suitable as a drug particularly in gene therapy.

L'invention concerne une préparation d'acide nucléique contenant moins de 1 % de protéine, de préférence moins de 0,1 % de protéine, dépourvue de bromure d'éthidium, de phénol de chlorure de césium et de détergents à base d'octylphénolpoly(éthylèneglycoléther)n, et présentant une teneur en endotoxines inférieure à 1 EU/mg d'ADN. Cette préparation s'utilise comme médicament, notamment en thérapie génique.

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