Methods of using il-21 for adoptive immunotherapy and...

A - Human Necessities – 61 – K

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A61K 35/14 (2006.01) C12N 5/078 (2010.01) C12N 5/0783 (2010.01) C12N 5/09 (2010.01) A61K 38/20 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2587136

Methods for preparing ex vivo T cell cultures using IL-21 compositions for use in adoptive immunotherapy are described. Addition of IL-21 to cultures of non- terminally differentiated T cells population, either isolated or present in peripheral blood mononuclear cells are exposed to one or more tumor antigens, and in the presence of IL-21 compositions and antigen presenting cells (APCs), the resulting T cell population has an enhanced antigen-specificity, and can be reintroduced into the patient. Methods are also disclosed for identifying tumor antigens by culturing T cell populations exposed to IL-21 compositions and APCs in the presence of tumor material.

L'invention concerne des méthodes de préparation ex vivo de cultures de lymphocytes T à l'aide de compositions d'IL-21 destinées à être utilisées en immunothérapie adoptive. Des cultures d'une population de lymphocytes T incomplètement différenciée, isolée ou présente dans des cellules mononucléaires du sang périphérique, dans lesquelles est ajoutée l'IL-21, sont exposées à un ou plusieurs antigènes tumoraux, et en présence de compositions d'IL-21 et de cellules présentatrices d'antigènes (APC), la population de lymphocytes T obtenue présente une spécificité antigénique améliorée et peut être réintroduite chez le patient. L'invention concerne également des méthodes d'identification d'antigènes tumoraux par culture de populations de lymphocytes T exposées à des compositions d'IL-21 et à des APC en présence de matière tumorale.

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