Methods for making recombinant proteins using apoptosis...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/67 (2006.01) C12N 15/12 (2006.01) C12N 15/34 (2006.01) C12N 15/57 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2384880

The invention provides improved methods of making and producing recombinant proteins in vitro cultures of host cells using apoptosis inhibitors. The use of one or more apoptosis inhibitors in the methods can reduce apoptosis in the cell cultures and markedly improve yield of the desired recombinant proteins.

L'invention concerne des méthodes améliorées servant à la fabrication et la production de protéines recombinantes dans des cultures in vitro de cellules hôtes à l'aide d'inhibiteurs d'apoptose. L'utilisation de un ou plusieurs inhibiteurs d'apoptose dans ces méthodes peut réduire l'apoptose dans des cultures de cellules et améliorer fortement le rendement des protéines recombinantes désirées.

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