G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 1/40 (2006.01) B01D 15/08 (2006.01) B01D 57/02 (2006.01) G01N 27/447 (2006.01) G01N 30/00 (2006.01)
Patent
CA 2511079
An arrangement for two-dimensional separation of a complex analyte mixture, e.g. of peptides, comprises a first separation device for the separation in a first dimension and a second device for the separation in the second dimension. In the area of the interface between the first and the second device a concentration zone is arranged for concentrating the individual sample fractions or components, respectively, leaving the first device before introduction into the second device. The concentration zone is defined in a segment between two or more electrodes seen in flow direction of the sample or sample fractions to be separated. The concentration is achieved by applying a voltage between two electrodes with polarity opposite to the charge of the components of the fraction, sufficiently strong to win the dragging force of the hydrodynamic flow. The introduction into the second device is carried out by turning off the potential difference between the electrodes.
F. Hoffmann-La Roche Ag
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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