C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01) C12N 5/00 (2006.01) C12Q 1/04 (2006.01)
Patent
CA 2074363
For the propagation or maintenance of cells in vitro, a culture medium which does not require CO2 in excess of the concentration found in ambient air is described. The medium contains the free base forms of L-arginine, L-cysteine, L-histidine, L-lysine and L-tyrosine; biotin; fatty acids; pyruvate; asparagine; low levels of sodium bicarbonate and is buffered with .beta.-glycerophosphate. Te use of the medium to culture tumor cells to test anti-tumor compounds is also disclosed.
Boyd Michael Ray
Monks Patricia Anne
Scudiero Dominic Albert
Skehan Philip James
Vistica David Thomas
Gowling Lafleur Henderson Llp
U.s. Departm Ent Of Commerce The United States Of America Represented By The Secretary
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