Method of converting water-soluble active proteins into...

C - Chemistry – Metallurgy – 12 – N

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C12N 11/02 (2006.01) C12N 9/76 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2629346

The present invention relates to a method of converting hydrophilic active proteins (HPiAP) into hydrophobic active proteins (HPoAP) suitable for the anchorage in their active form on hydrophobic substrates. The present invention also relates to the preparation of ordered monomolecular layers of oriented active proteins immobilized onto hydrophobic solid supports to be used for mechanical manipulation and investigations, including Atomic Force Microscopy (AFM) in aqueous solutions and assays employing the same devices .

La présente invention concerne un procédé de conversion de protéines actives hydrophiles (HPiAP) en protéines actives hydrophobes (HPoAP) convenant pour l'ancrage de leur forme active sur des substrats hydrophobes. La présente invention concerne également la préparation de couches monomoléculaires ordonnées de protéines actives orientées immobilisées sur des supports solides hydrophobes à utiliser pour des recherches et la manipulation mécaniques, dont la microscopie à force atomique (AFM) dans des solutions aqueuses et des essais employant ces appareils.

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