Specific and high affinity binding proteins comprising...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/435 (2006.01)

Patent

CA 2661160

The present invention relates to a recombinant binding protein comprising at least one derivative of the Src homology 3 domain (SH3) of the FYN kinase, wherein at least one amino acid in or positioned up to two amino acids adjacent to the src loop and/or at least one amino acid in or positioned up to two amino acids adjacent to the RT loop is substituted, deleted or added. Furthermore, the invention is directed to fusion proteins comprising a binding protein according to the invention fused to a pharmaceutically and/or diagnostically active component. In addition, the invention concerns nucleotides coding for these binding and/or fusion proteins as well as corresponding vectors and host cells. Last but not least, the present invention relates to the use of binding and/or fusion proteins of the present invention for preparing a medicament or a diagnostic means as well as to pharmaceutical or diagnostic compositions comprising said binding and/or fusion proteins.

La présente invention concerne une protéine recombinante de liaison qui comprend au moins un dérivé du domaine 3 d'homologie Src (SH3) de la kinase FYN, dans laquelle au moins un acide aminé dans ou positionné jusqu'à deux acides aminés adjacents à la boucle src et/ou au moins un acide aminé dans ou positionné jusqu'à deux acides aminés adjacents à la boucle RT est substitué, supprimé ou ajouté. En outre, l'invention concerne des protéines de fusion comprenant une protéine de liaison selon l'invention fusionnée à un composant pharmaceutiquement et/ou diagnostiquement actif. De plus, l'invention concerne des nucléotides qui codent ces protéines de liaison et/ou de fusion et également les cellules hôtes et les vecteurs correspondants. Dernier aspect mais pas des moindres, la présente invention concerne l'utilisation des protéines de liaison et/ou de fusion de la présente invention pour préparer un médicament ou un moyen de diagnostic et également des compositions pharmaceutiques ou de diagnostic qui comprennent lesdites protéines de liaison et/ou de fusion.

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