Rapid extraction of hair root dna

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01) C12N 5/071 (2010.01) A61K 35/36 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2359992

DNA-containing hair root cells are liberated from hair samples by soaking hair roots ends of the hair shafts in an aqueous solution consisting essentially of dithiothreitol, a surfactant such as sodium dodecylsulfate, a chelate such as ethylenediamine tetraacetic acid, an inorganic salt (NaCI), tris (hydroxymethyl) aminomethane and sufficient hydrochloric acid to make the pH of the solution approximately 8.

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