Temporal or spatial characterization of biosynthetic events...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2559095

The invention provides methods useful for establishing timing or spatial location of a biosynthetic event in a living organism, without disrupting the event of interest and without disrupting the living organism. A temporal or spatial gradient of an isotopically labeled biochemical precursor is created, which serves to isotopically fingerprint (i.e., definitively mark) when or where biosynthesis occurs. The methods of the invention are broadly applicable to a variety of medical, public health, and diagnostic applications, as well as for establishing sequences of biochemical events that occur within a living organism.

Cette invention concerne des procédés utilisés pour établir le moment et l'emplacement spatial d'un événement de biosynthèse dans un organisme vivant, sans perturber ledit événement ni ledit organisme vivant. Un gradient temporel ou spatial d'un précurseur biochimique présentant un marquage isotope est crée. Ce gradient permet d'établir des empreintes isotopiques (c'est-à-dire, de marquer définitivement) au moment ou à l'endroit où intervient la biosynthèse. Les procédés décrits dans cette invention peuvent être largement appliqués à une vaste gammes d'applications médicales, de santé publique, et diagnostiques, ainsi que pour établir des séquences d'événements biochimiques se produisant dans un organisme vivant.

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